爬片(pian)又(you)名(ming)細胞爬片(pian),是(shi)指讓(rang)玻片(pian)浸(jin)在(zai)細胞培養(yang)基(ji)內,細胞在(zai)玻片(pian)上(shang)生長,主要(yao)用(yong)於(yu)組(zu)織(zhi)學(xue),免(mian)疫組(zu)織(zhi)化(hua)學(xue),bing凍切片(pian),細胞塗片(pian),原(yuan)位(wei)雜(za)交(jiao)等。
細胞爬片(pian)保(bao)存(cun):
細胞爬片(pian)有(you)三(san)層(ceng)包裝,在(zai)超凈(jing)臺(tai)無菌(jun)的(de)狀態下打開(kai)包裝.未用完的(de)爬片(pian)按(an)原(yuan)先(xian)包裝方式(shi)再包裝起(qi)來(lai)即(ji)可(ke).
細胞爬片(pian)特(te)點(dian):
1.玻片(pian)表面(mian)經過(guo)TC處理,雙面(mian)均可使用(yong).
2.γ射線(xian)滅菌(jun),保(bao)證(zheng)無菌(jun).
3.使(shi)用便捷,只需打開(kai)包裝,將爬片(pian)放(fang)入孔板(ban)內,將(jiang)細胞懸液(ye)滴(di)到(dao)爬片(pian)上(shang)即(ji)可培養(yang).
4.爬片(pian)厚度(du)為0.17mm,直徑為8mm/14mm/20mm/25mm.分(fen)別適(shi)用(yong)於(yu)48孔細胞培養(yang)板(ban)/24孔細胞培養(yang)板(ban)/12孔細胞培養(yang)板(ban)/6孔細胞培養(yang)板(ban)
5.*吸附(fu):該(gai)玻片(pian)表面(mian)具(ju)有(you)yong久(jiu)的(de)陽離(li)子(zi)電荷(he),可(ke)以(yi)通(tong)過(guo)靜電作(zuo)用吸附冰(bing)凍切片(pian)組(zu)織(zhi)或(huo)細胞,使之(zhi)粘(zhan)附在(zai)玻片(pian)上(shang),進(jin)而(er)在(zai)玻璃(li)和(he)切片(pian)組(zu)織(zhi)之(zhi)間(jian)形成(cheng)共價鍵(jian).因此,無需粘(zhan)黏劑或(huo)者蛋(dan)白(bai)包被,該(gai)玻片(pian)也能牢固(gu)的(de)粘(zhan)附切(qie)片(pian)或(huo)細胞。
操(cao)作(zuo)註意事項:
使用細胞爬片(pian)時(shi)(比(bi)如(ru)在(zai)六孔(kong)板中放(fang)入爬片(pian),六孔(kong)板的(de)孔底面(mian)積(ji)往(wang)往(wang)會(hui)比(bi)爬片(pian)面(mian)積(ji)多出(chu)壹部分(fen),加細胞懸液(ye)時常(chang)常(chang)就是(shi)細胞鋪滿(man)整個孔底,有時(shi)細胞生長邊(bian)集現(xian)象(xiang),就是(shi)靠近(jin)壁邊(bian)緣密度(du)要(yao)高(gao)些(xie),這(zhe)樣處於(yu)玻片(pian)上(shang)爬的細胞數量(liang)就可能相(xiang)對較少(shao))
比(bi)較好的做法(fa)是(shi)將玻片(pian)放(fang)入孔板(ban)的孔(kong)內(nei)後,加細胞懸液(ye)時只(zhi)滴(di)到(dao)玻片(pian)上(shang),壹(yi)直滴(di)到(dao)液(ye)體布(bu)滿(man)整個玻片(pian)又(you)不(bu)會(hui)溢(yi)出(chu)玻片(pian)邊(bian)緣(因為液(ye)體表面(mian)張力(li)作用可(ke)以(yi)很容(rong)易做到(dao)這(zhe)壹(yi)點(dian)。),放(fang)在(zai)培養(yang)箱(xiang)裏,等(deng)細胞貼(tie)壁後(hou),取出,再滴(di)加(jia)培(pei)養(yang)基(ji)布(bu)滿(man)整個孔(kong)底(di),繼續培養(yang),這(zhe)樣玻片(pian)上(shang)的(de)細胞生長的(de)密(mi)度(du)就會很集中.